2026-06-08T13:40:19Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/135482023-07-17T21:26:33Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Torres-Sánchez, María author Creevey, Christopher J. author Kornobis, Etienne author Gower, David J. author Wilkinson, Mark author San Mauro, Diego 2023-06-17T13:27:51Z 2023-06-17T13:27:51Z 2019-02-01 1756-1663 10.1093/dnares/dsy034 https://hdl.handle.net/20.500.14352/13548 https://www.ncbi.nlm.nih.gov/pubmed/30351380 RNA sequencing (RNA-seq) has become one of the most powerful tools to unravel the genomic basis of biological adaptation & diversity. Although challenging, RNA-seq is particularly promising for research on non-model, secretive species that cannot be observed in nature easily and therefore remain comparatively understudied. Among such animals, the caecilians (order Gymnophiona) likely constitute the least known group of vertebrates, despite being an old and remarkably distinct lineage of amphibians. Here, we characterize multi-tissue transcriptomes for five species of caecilians that represent a broad level of diversity across the order. We identified vertebrate homologous elements of caecilian functional genes of varying tissue specificity that reveal a great number of unclassified gene families, especially for the skin. We annotated several protein domains for those unknown candidate gene families to investigate their function. We also conducted supertree analyses of a phylogenomic dataset of 1,955 candidate orthologous genes among five caecilian species and other major lineages of vertebrates, with the inferred tree being in agreement with current views of vertebrate evolution and systematics. Our study provides insights into the evolution of vertebrate protein-coding genes, and a basis for future research on the molecular elements underlying the particular biology and adaptations of caecilian amphibians. eng Atribución-NoComercial 3.0 España Multi-tissue transcriptomes of caecilian amphibians highlight incomplete knowledge of vertebrate gene families journal article URL https://docta.ucm.es/bitstreams/fb79e8b6-8a29-4c11-baa6-bd1479bb8b1c/download File MD5 07c4b07436bf5280bb38c2e281c0d715 503636 application/pdf Torres-Sanchez-DBEE-Multi-tissue.pdf