2026-08-24T05:18:59Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1366212026-05-08T23:54:28Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Perelló, Alberto author Smoglica, Camilla author González-Crespo, Carlos author Pérez Sancho, Marta author González Barrio, David author Herranz Benito, Carmen author Dashti, Alejandro author Sánchez, Sergio author Carmena, David author Varzandi, Amir Reza author Ferroglio, Ezio author Domínguez Rodríguez, Lucas José author Martínez-López, Beatriz author Gortázar, Christian author 2026 The wildlife-livestock-environment interface is a complex system with implications for biodiversity and diseases. Environmental nucleic acid detection (ENAD) is a non-invasive method for monitoring DNA/RNA from microorganisms, meiofauna, and hosts. However, how environmental variables influence ENAD in heterogeneous geographic contexts remains poorly explored. In this study, 18 sites were evaluated in Iberian Peninsula, collecting 10 surface sponge samples per site and a total of 146 environmental fecal samples to investigate how environmental, mammal community and wildlife health variables may influence ENAD, using different pathogen-related markers as the study model. Differences in ENAD were assessed among sponge sampling methods and between sponge and fecal samples. The results show that environmental characteristics influence ENAD, at least for pathogen-related markers and at larger geographic scales. Greater pathogen marker diversity and richness was observed at higher latitudes. Most markers found in feces were also detectable in surface sponges. Combining different sponge sampling methodologies provided the best overall coverage of detectable environmental DNA. A predictive map linking ENAD in sponges to environmental factors was developed Perelló, A., Smoglica, C., González-Crespo, C., Pérez-Sancho, M., González-Barrio, D., Herranz, C., Dashti, A., Sánchez, S., Carmena, D., Varzandi, A. R., Ferroglio, E., Domínguez, L., Martínez-López, B., & Gortázar, C. (2026). Tracking pathogen-related markers with eDNA in natural areas: how environmental factors shape surveillance strategies. Veterinary research, 10.1186/s13567-026-01746-6. Advance online publication. https://doi.org/10.1186/s13567-026-01746-6 0928-4249 10.1186/s13567-026-01746-6 https://hdl.handle.net/20.500.14352/136621 1297-9716 https://doi.org/10.1186/s13567-026-01746-6 42050596 https://pubmed.ncbi.nlm.nih.gov/42050596/ Tracking pathogen-related markers with eDNA in natural areas: how environmental factors shape surveillance strategies