2026-06-29T16:49:22Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1368962026-05-26T00:00:51Zcom_20.500.14352_14col_20.500.14352_15

Development and characterisation of a radiobiology proton beamline using radiochromic film dosimetry Marques Coelho, Carina de la Fuente Fernández, Pablo Bononad, Paula Viñals, Sílvia Tavares de Sousa, Célia Galaviz, Daniel Herrera, Federico del Monte-García, Inés Sánchez Parcerisa, Daniel Cortés-Llanos, Belén Objective. To develop and optimise a dedicated low-energy proton beamline at the Centre for Micro-Analysis of Materials (CMAM, Madrid, Spain) for radiobiological applications. Approach. An automated irradiation system was implemented, integrating Gafchromic EBT3 radiochromic film dosimetry corrected for linear energy transfer dependent quenching and post-irradiation darkening. Dosimetric calibration was performed using multichannel analysis, and beam performance was systematically evaluated as a function of distance, raster scanning area, beam intensity and reproducibility. Main results. Optimal operating conditions were identified at moderate beam currents (⩽1nA) and scanning areas of 40 × 40 to 50 × 50mm2, yielding homogeneous dose distributions with reproducibility better than 8%. The dosimetric protocol demonstrated linearity across clinically relevant dose ranges and allowed a reliable correlation between irradiation parameters and absorbed dose. Proof-of-concept experiments on U-87 MG glioblastoma cells confirmed the system’s ability to deliver controlled and biologically effective proton exposures, as demonstrated by clonogenic survival assays. Significance. These results establish the CMAM implantation beamline as a robust and versatile platform for preclinical proton radiobiology, providing accurate dosimetric control and supporting investigations of relative biological efficacy. The system facilitates translational advances in proton radiobiology, bridging physical and biological studies in low-energy proton irradiation. 2026-05-25T07:13:18Z 2026-05-25T07:13:18Z 2026-05-25T07:13:18Z 2026-04-15 journal article https://hdl.handle.net/20.500.14352/136896 0031-9155 10.1088/1361-6560/ae5457 1361-6560 eng info:eu-repo/grantAgreement/FCT/ProtoTera/PRT/BD/151545/2021/PT/The impact of radiation therapy in protein aggregation/PRT/BD/151545/2021 info:eu-repo/grantAgreement/FCT/LA/P/0016/2020/PT/Laboratório de Instrumentação e Física Experimental de Partículas/LIP info:eu-repo/grantAgreement/FCT/UID/04046/2025/PT/Instituto de Biosistemas e Ciências Integrativas Centre grant/BioISI info:eu-repo/grantAgreement/EC/HE/101060008/EU/eurOpean platForm For accEssing nucleaR R&d facilities/OFFERR info:eu-repo/grantAgreement/Comunidad de Madrid/Biomedicina 2022/S2022/BMD-7434/ES/Advanced Strategies and new Approaches for Protontherapy/ASAP-CM info:eu-repo/grantAgreement/UCM/Plan UCM-Santander 2021/PR27/21-014/ES/Farmacocinética PET no invasiva para estudios clínicos y preclínicos/PR27/21-014 info:eu-repo/grantAgreement/AEI/Plan Estatal de I+D+i 2021-2023/PLEC2022-009256/ES/Comprendiendo la radioterapia FLASH con dispositivos de tejido-en-chip y resonancia magnética mejorada con hiperpolarización/FLASHOnChip info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141080OB-C22/ES/NANOTERAGNOSIS ESTIMULADA MAGNETICAMENTE PARA MODELO Y TERAPIA DEL ICTUS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-142417OB-I00/ES/RECUBRIMIENTOS PARA INSTRUMENTACION ESPACIAL Y FOTONICA EN EL UV Y UV LEJANO: NANOESTRUCTURA Y OPTIMIZACION INCLUYENDO IONES DE ALTA ENERGIA/ info:eu-repo/grantAgreement/AEI/Consolidación Investigadora 2024/CNS2024-154729/ES/Ampliando los límites de la terapia de protones con herramientas de nanotecnología/CNS2024-154729 info:eu-repo/grantAgreement/CMAM-UAM/BeamTime/IMP007/23/ES/Development and characterisation of a radiobiology proton beamline/IMP007/23 info:eu-repo/grantAgreement/CMAM-UAM/BeamTime/IMP020/24/ES/Development and characterisation of a radiobiology proton beamline/IMP020/24 info:eu-repo/grantAgreement/IAEA/CRP/F11024/INT/Improving Radiation Biology for Particle Therapy/F11024 Marques Coelho, Carina, et al. «Development and characterisation of a radiobiology proton beamline using radiochromic film dosimetry». Physics in Medicine & Biology, vol. 71, n.o 8, abril de 2026, p. 085003. DOI.org (Crossref), https://doi.org/10.1088/1361-6560/ae5457. http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International