2026-06-29T16:48:41Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/1369072026-05-26T00:17:48Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Didkowska, Anna author Navarro Gómez, Alejandro author Dorrego Rodríguez, Abel author Martínez, Jorge author Martínez Alares, Irene author Kloch, Marta author González Domínguez, Sergio author Olech, Wanda author Cruz López, Fátima author Anusz, Krzysztof author García Benzaquén, Nerea 2026-05-25T11:04:05Z 2026-05-25T11:04:05Z 2026 Didkowska, A., Navarro, A., Dorrego, A., Martínez, J., Martínez, I., Kloch, M., González, S., Olech, W., Cruz-Lopez, F., Anusz, K., & García, N. (2026). Serological and Molecular Detection of Zoonotic Pathogens in European Bison (Bison bonasus) and Associated Ticks from Poland. Pathogens, 15(6), 562. https://doi.org/10.3390/pathogens15060562 10.3390/pathogens15060562 https://hdl.handle.net/20.500.14352/136907 2076-0817 https://doi.org/10.3390/pathogens15060562 As wild ungulates, including European bison, increasingly share habitats with livestock, surveillance of infectious zoonotic agents in their populations is essential for both wildlife and public health. This study aimed to screen for selected zoonotic pathogens in European bison from Poland. Samples (blood, ticks, and spleen) were collected from 86 animals. Serum was used for serological testing using commercial ELISA kits for Borrelia burgdorferi sensu lato, Brucella spp., and hepatitis E virus (HEV); ticks were analysed by real-time PCR targeting B. burgdorferi s.l., Anaplasma phagocytophilum, and Brucella spp., and spleen samples from Brucella-seropositive animals were cultured. Serological analysis revealed that 53.9% of European bison were seropositive for B. burgdorferi s.l., while 25.3% showed seroreactivity against Brucella spp.; however, these findings were not supported by molecular or culture confirmation, suggesting possible non-specific reactions or past exposure. No serum samples were positive for HEV antibodies, and no Brucella spp. were isolated from spleen samples. Molecular analysis of ticks detected B. burgdorferi s.l. DNA in 4.8% of amples and sequencing confirmed Borrelia garinii in one case. In contrast, A. phagocytophilum DNA was detected in 59.0% of ticks. No ticks tested positive for Brucella DNA. These findings indicate substantial exposure of European bison to tick-borne pathogens, particularly B. burgdorferi s.l. and A. phagocytophilum. However, Brucella seropositivity should be interpreted with caution due to the lack of molecular or culture confirmation. eng Attribution 4.0 International Serological and Molecular Detection of Zoonotic Pathogens in European Bison (Bison bonasus) and Associated Ticks from Poland journal article 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URL https://docta.ucm.es/bitstreams/2082a9fa-a784-4880-90da-802e8cc40563/download File MD5 6b39dc905981e5310eb57b3c26580d24 735346 application/pdf pathogens-15-00562.pdf