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oai:docta.ucm.es:20.500.14352/1377442026-06-26T00:11:55Zcom_20.500.14352_14col_20.500.14352_15

Autohydrolysis and microwave ionic liquid pretreatment of Pinus radiata: Imaging visualization and analysis to understand enzymatic digestibility Rigual Hernández, Victoria de los Ángeles Domínguez Toribio, Juan Carlos Santos, Tamara Rivas Siota, Sandra Alonso Rubio, María Virginia Oliet Pala, María Mercedes Rodríguez Somolinos, Francisco 66 Softwood Autohydrolysis Ionic liquid Surface fractal dimension Enzymatic hydrolysis Ingeniería química 3303 Ingeniería y Tecnología Químicas Sequential pretreatments provide advantages of every single process towards a complete biomass fractionation. In this work, autohydrolysis and IL microwave pretreatments are sequentialy studied, and their effect to enhance enzymatic hydrolysis is analyzed. Mild, intermediate and severe autohydrolysis are combined with four IL temperatures (50, 80, 120 and 150 °C). Pretreated solids are enzymatically hydrolyzed and compared in terms of chemical composition and morphology. Digital image analysis is employed to numerically determine the heterogeneity of the solids using surface fractal dimension and lacunarity parameters. In this study, the negative effect of intermediate and severe autohydrolysis, over the subsequent IL pretreatment is demonstrated. Mild autohydrolysis and high IL conditions (AH150IL120) results in digestibilities of 78.8 g of glucan/100 g of glucan introduced. High surface fractal values (in the range of 2.5461–2.7124) and low lacunarities (0.0818–0.2563) enhance the enzymatic accessibility of pine wood. Furthermore, the negative effect of softwood lignin accumulation in the surface is observed using confocal fluorescence microscopy. Keywords: Softwood; Autohydrolysis; Ionic liquid; Surface fractal dimension; Enzymatic hydrolysis Ministerio de Economía y Competitividad Depto. de Ingeniería Química y de Materiales Fac. de Ciencias Químicas TRUE pub 2026-06-25T07:10:25Z 2026-06-25T07:10:25Z 2019 journal article VoR https://hdl.handle.net/20.500.14352/137744 XXXX-XXXX 10.1016/j.indcrop.2019.03.068 eng info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-88623-R/ES/VALORIZACION DE FRACCIONES LIGNOCELULOSICAS OBTENIDAS POR BIORREFINO DE ESPECIES MADERERAS MEDIANTE PROCESOS IONOSOLV Y ORGANOSOLV/ Victoria Rigual, Juan C. Domínguez, Tamara M. Santos, Sandra Rivas, M. Virginia Alonso, Mercedes Oliet, Francisco Rodriguez, Autohydrolysis and microwave ionic liquid pretreatment of Pinus radiata: Imaging visualization and analysis to understand enzymatic digestibility, Industrial Crops and Products, Volume 134, 2019, Pages 328-337, ISSN 0926-6690, https://doi.org/10.1016/j.indcrop.2019.03.068. (https://www.sciencedirect.com/science/article/pii/S0926669019302341) embargoed access application/pdf Elsevier