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00925njm 22002777a 4500 dc Rigual Hernández, Victoria de los Ángeles author Domínguez Toribio, Juan Carlos author Alonso Rubio, María Virginia author Oliet Pala, María Mercedes author Rodríguez Somolinos, Francisco author Santos, Tamara author 2018 Differences in hardwood and softwood, elucidates their behaviour against pretreatments varies. In this work, microwave ionic liquid (IL) and autohydrolysis (AH) pretreatments were applied to Eucalyptus globulus (as a model of hardwood) and Pinus radiata (as a model of softwood). The comparison between hardwood and softwood of microwave ionic liquid (IL) and autohydrolysis (AH) were evaluated in terms of chemical composition of pretreated solids, liquid by streams composition (hemicellulose and lignin extraction) and, substrates enzymatic digestibility. Furthermore, micrographs using scanning electron microscopy (SEM) and confocal fluorescence microscopy supported results obtained. In this study, it has been demonstrated that autohydrolysis pretreatment effectiveness, through maximizing enzymatic digestibility, is opposite in hardwood (73 g glucan/100 g glucan introduced at severe conditions) and softwood (10 g/100 g glucan). IL pretreatment has been especially effective in softwood with higher digestibilities (78 g glucan/100 g glucan introduced) than those obtained in hardwood (68 g glucan/100 g glucan introduced). Confocal fluorescence microscopy images, together with SEM images have resulted to be a clarifying technique to explain enzymatic digestibility results. Final sugars yields after the whole process have shown that low solid yields recoveries obtained in AH treatments have considerably worsened final glucose production, mainly in softwood. IL microwave pretreatment have resulted in higher glucose yields in softwood than in hardwood. Victoria Rigual, Tamara M. Santos, Juan Carlos Domínguez, M. Virginia Alonso, Mercedes Oliet, Francisco Rodriguez, Evaluation of hardwood and softwood fractionation using autohydrolysis and ionic liquid microwave pretreatment, Biomass and Bioenergy, Volume 117, 2018, Pages 190-197, ISSN 0961-9534, https://doi.org/10.1016/j.biombioe.2018.07.014. (https://www.sciencedirect.com/science/article/pii/S0961953418301831) 10.1016/j.biombioe.2018.07.014 https://hdl.handle.net/20.500.14352/137745 https://doi.org/10.1016/j.biombioe.2018.07.014 https://www.sciencedirect.com/science/article/pii/S0961953418301831 Evaluation of hardwood and softwood fractionation using autohydrolysis and ionic liquid microwave pretreatment