2026-08-22T00:37:59Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/1378792026-06-30T23:58:01Zcom_20.500.14352_14col_20.500.14352_15

Butyrate-Dependent Conformational Switch Promotes Nuclear Translocation of the Mustard Allergen Sin a 1 in Human Gut Epithelial Cells. García Gordo, Rubén Parrón Ballesteros, Jorge Olmo López, Nieves Villalba Díaz, María Teresa Batanero Cremades, Eva Turnay Abad, Francisco Javier Sin a 1, the major allergen from mustard seeds, is a highly stable 2S albumin capable of reaching the intestinal epithelium in its intact form. In this study, we investigated whether butyrate, a microbiota-derived short-chain fatty acid, directly interacts with Sin a 1 and modulates its cellular behavior in Caco-2 cells. Circular dichroism analyses revealed that short-chain fatty acids induce conformational changes in Sin a 1. However, fluorescence spectroscopy showed that only butyrate modified the tertiary structure of the light chain, exposing a putative nuclear localization signal and thereby promoting the importin-dependent nuclear translocation of the allergen. In the absence of butyrate, Sin a 1 remained predominantly in the cytoplasm. In addition, butyrate altered NFKB1 transcriptional responses in Sin a 1-treated cells. Our findings reveal an effect of butyrate on the intracellular trafficking of allergens that had not previously been described, suggesting a novel mechanism by which microbiota-derived metabolites may influence food allergenicity. 2026-06-30T09:01:25Z 2026-06-30T09:01:25Z 2026-06-30T09:01:25Z 2026-06-24 journal article https://hdl.handle.net/20.500.14352/137879 XXXX-XXXX https://doi.org/10.1021/acs.jafc.6c01248 1520-5118 eng PID2020-116692RBI00 Butyrate-Dependent Conformational Switch Promotes Nuclear Translocation of the Mustard Allergen Sin a 1 in Human Gut Epithelial Cells. Rubén G. Gordo, Jorge Parrón-Ballesteros, Nieves Olmo, Mayte Villalba, Eva Batanero, and Javier Turnay Journal of Agricultural and Food Chemistry 2026 74 (24), 19053-19065 DOI: 10.1021/acs.jafc.6c01248 http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International ACS