2026-06-29T02:29:38Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/137902024-06-20T16:20:30Zcom_20.500.14352_14col_20.500.14352_15
Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production
Guevara Acosta, Flor Govinda
Olortegui Flores, Yamileth
Fernández de las Heras, Laura
Perera González, Julián
Navarro Llorens, Juana María
Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.
2023-06-17T13:34:43Z
2023-06-17T13:34:43Z
2023-06-17T13:34:43Z
2019-07-26
journal article
https://hdl.handle.net/20.500.14352/13790
1932-6203
https:// doi.org/10.1371/journal.pone.0220492
eng
(RTC2014-2249-1)
info:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación//RTC2014-2249-1
Guevara G, Olortegui Flores Y, Fernández de Las Heras L, Perera J, Navarro Llorens JM. Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production. PLoS One. 2019 Jul 26;14(7):e0220492. doi: 10.1371/journal.pone.0220492. PMID: 31348804; PMCID: PMC6660089.
http://creativecommons.org/licenses/by-nc-nd/4.0/
open access
Attribution-NonCommercial-NoDerivatives 4.0 International
Public Library of Science