2026-06-29T08:07:13Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/175762024-09-23T17:20:58Zcom_20.500.14352_14col_20.500.14352_15
Characterization of a new toxin from the entomopathogenic
fungus Metarhizium anisopliae: the ribotoxin anisoplin
Olombrada, Miriam
Medina, Pilar
Budia, Flor
Gavilanes, José
Martínez Del Pozo, Álvaro
García Ortega, Lucía
577.1
577.2
hirsutellin
insecticide
ribonuclease
ribotoxin
sarcin.
Biología molecular (Biología)
Bioquímica (Biología)
2415 Biología Molecular
2302 Bioquímica
Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against target ribosomes and suggested as potential insecticides. Here, we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are on perspective.
Ministerio de Economía y Competitividad (MINECO)
Universidad Complutense de Madrid
Depto. de Bioquímica y Biología Molecular
Fac. de Ciencias Químicas
FALSE
pub
2023-06-17T21:48:47Z
2023-06-17T21:48:47Z
2017-01-01
journal article
https://hdl.handle.net/20.500.14352/17576
1431-6730 (Print), 1437-4315 (Online)
10.1515/hsz-2016-0119
eng
BFU2012-32404
AGL2010-22196-C02-02
ESFUNPROT-UCM
open access
application/pdf
De Gruyter