2026-08-21T01:51:39Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/176842023-08-28T05:21:49Zcom_20.500.14352_14col_20.500.14352_15
Lactoferrin Levels in Tears are Increased by the Topical Application of Diadenosine Tetraphosphate
Loma Lozano, Patricia
Guzmán Aránguez, Ana Isabel
Pérez de Lara, María Jesús
Pintor, Jesús
617.764
577.15
Diadenosine tetraphosphate
Lactoferrin
Purinergic receptors
Tear film
Bioquímica (Medicina)
Anatomía ocular
Received 20 July 2015 ; Revised 8 October 2015 ; Accepted 18 October 2015
Purpose: This study was undertaken to determine the effect of the topical application of diadenosine tetraphosphate on lactoferrin levels in rabbit tears.
Methods: Diadenosine tetraphosphate was topically instilled in a single-dose, tear samples were collected by micropipette and lactoferrin was measured by Enzyme-Linked ImmunoSorbent Assay (ELISA).
Results: The concentration of lactoferrin in rabbit tears was significantly increased 1 h after diadenosine tetraphosphate application, remaining elevated for 3 h more. This effect was blocked by P2 receptors antagonists.
Conclusions: Topical application of diadenosine tetraphosphate stimulates the secretion of lactoferrin in rabbit tears through P2 receptor activation.
Ministerio de Ciencia e Innovación (MICINN)
RETICS (Redes Temáticas de Investigación Cooperativa en Salud)
Unidad Docente de Bioquímica y Biología Molecular
Fac. de Óptica y Optometría
TRUE
pub
2023-06-17T21:52:07Z
2023-06-17T21:52:07Z
2016-09
journal article
https://hdl.handle.net/20.500.14352/17684
0271-3683
10.3109/02713683.2015.1113431
eng
SAF2013-44416-R
RD12/0034/0003
restricted access
application/pdf
Taylor & Francis