2026-06-27T10:29:29Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/178622024-10-02T17:58:58Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author López Rodríguez, Juan Carlos author Barderas Manchado, Rodrigo author Echaide Torreguitar, Mercedes author Pérez-Gil, Jesús author Villalba, Mayte author Batanero Cremades, Eva author Cruz Rodríguez, Antonio 2023-06-17T21:58:17Z 2023-06-17T21:58:17Z 2016-10-25 0743-7463, ESSN: 1520-5827 10.1021/acs.langmuir.6b02831 https://hdl.handle.net/20.500.14352/17862 http://pubs.acs.org/doi/abs/10.1021/acs.langmuir.6b02831 Aeroallergens are airborne substancesmainly proteinscapable of triggering Th2-immune responses in respiratory allergies. They enter into the body through the upper airways, reaching the mucosa afterward. Mucosae lining at the luminal side consists of an epithelial barrier completely covered by mucus and pulmonary surfactant. Both pulmonary surfactant and plasma membrane of the epithelial cells represent two physiological phospholipid-based barriers where allergens first impact before triggering their biological effects. The interaction of allergens with lipids at relevant physiological surfaces could promote structural changes on the molecule, resulting on a potential modification of its allergenic properties. In this work, we have first described the surface and phospholipid interaction capabilities of the clinically relevant aeroallergen Ole e 1, the main allergen of olive tree pollen. By using epifluorescence microscopy of Langmuir transferred films, we observed that lipid-packed ordered domains may function as a preferential location for allergen to accumulate at the air−liquid interface, an effect that is abolished in the presence of cholestenone. The possible implications of phospholipid-interfacial effects in the modification of allergen structural and functional properties will be discussed eng Surface activity as a crucial factor of the biological actions of Ole e1, the main aeroallergen of olive tree (Olea europaea) pollen journal article URL https://docta.ucm.es/bitstreams/fdff7767-c08b-428d-80c4-7593d3144bc1/download File MD5 d5ee2b0102aed8c9785b6380e02161d8 2796890 application/pdf López- rodríguez, J. C. Surface activity as a crucial.pdf URL https://docta.ucm.es/bitstreams/ba26a332-ce5b-40ad-b4e6-a175c00f2cec/download File MD5 44d58af9e9904f2765f37a3b132b0e45 40714 text/plain López- rodríguez, J. C. Surface activity as a crucial.pdf.txt