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oai:docta.ucm.es:20.500.14352/179252024-09-16T15:50:44Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Valencia Mahón, Jaris author Jiménez Pérez, Eva author Martínez, Víctor G. author Amo, Beatriz G. del author Hidalgo, Laura author Entrena Martínez, Ana author Martínez Fernández De Sevilla, Lidia author Río Gallegos, Francisco José Del author Varas Fajardo, Alberto author Vicente López, María Ángeles author Sacedón Ayuso, Rosa author 2017 Fibroblastic reticular cells (FRCs) are essential players during adaptive immune responses not only as a structural support for the encounter of antigen-presenting cells and naive T lymphocytes but also as a source of modulatory signals. However, little is known about this cell population in humans. To address the phenotypical and functional analysis of human FRCs here we established splenic (SP) and mesenteric lymph node (LN) CD45- CD31- CD90+ podoplanin+ myofibroblastic cell cultures.They shared the phenotypical characteristics distinctive of FRCs, including the expression of immunomodulatory factors and peripheral tissue antigens. Nevertheless, human FRCs also showed particular features, some differing from mouse FRCs, like the lack of nitric oxide synthase (NOS2) expression after interferon (IFN)γstimulation. Interestingly, SP-FRCs expressed higher levels of interleukin (IL)-6, BMP4, CCL2, CXCL12 and Notch molecules, and strongly adapted their functional profile to lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (Poly I:C) and IFNγ stimulation. In contrast, we found higher expression of transforming growth factor (TGF)β and Activin A in LN-FRCs that barely responded via Toll-Like Receptor (TLR)3 and constitutively expressed retinaldehyde dehydrogenase 1 enzyme, absent in SP-FRCs. This study reveals human FRCs can be valuable models to increase our knowledge about the physiology of human secondary lymphoid organs in health and disease and to explore the therapeutic options of FRCs. Valencia Mahón, J., Jiménez Pérez, E., Martínez, V. G. et al. «Characterization of Human Fibroblastic Reticular Cells as Potential Immunotherapeutic Tools». Cytotherapy, vol. 19, n.o 5, mayo de 2017, pp. 640-53. DOI.org (Crossref), https://doi.org/10.1016/j.jcyt.2017.01.010. 1465-3249 10.1016/j.jcyt.2017.01.010 https://hdl.handle.net/20.500.14352/17925 https//doi.org/10.1016/j.jcyt.2017.01.010 http://www.tandfonline.com/toc/icyt20/current Characterization of human fibroblastic reticular cells as potential immunotherapeutic tools