2026-09-22T00:45:42Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/180082023-08-26T15:29:04Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Blanco Imperiali, Ayelén M.
author
Bertucci, Juan I.
author
Valenciano González, Ana Isabel
author
Delgado, María Jesús
author
Unniappan, Suraj
2023-06-17T22:03:58Z
2023-06-17T22:03:58Z
2017-07
0018-506X
10.1016/j.yhbeh.2017.05.004
https://hdl.handle.net/20.500.14352/18008
https://www.journals.elsevier.com/hormones-and-behavior/
Ghrelin is an important gut-derived hormone with an appetite stimulatory role, while most of the intestinal hormones, including cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1), are appetite-inhibitors. Whether these important peptides with opposing roles on food intake interact to regulate energy balance in fish is currently unknown. The aim of this study was to characterize the putative crosstalk between ghrelin and CCK, PYY and GLP-1 in goldfish (Carassius auratus). We first determined the localization of CCK, PYY and GLP-1 in relation to ghrelin and its main receptor GHS-R1a (growth hormone secretagogue 1a) in the goldfish intestine by immunohistochemistry. Colocalization of ghrelin/GHS-R1a and CCK/PYY/GLP-1 was found primarily in the luminal border of the intestinal mucosa. In an intestinal explant culture, a significant decrease in prepro-cck, prepro-pyy and proglucagon transcript levels was observed after 60 min of incubation with ghrelin, which was abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6 (except for proglucagon). The protein expression of PYY and GLP-1 was also downregulated by ghrelin. Finally, intraperitoneal co-administration of CCK, PYY or GLP-1 with ghrelin results in no modification of food intake in goldfish. Overall, results of the present study show for the first time in fish that ghrelin exerts repressive effects on enteric anorexigens. It is likely that these interactions mediate the stimulatory effects of ghrelin on feeding and metabolism in fish.
eng
Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus)
journal article
URL
https://docta.ucm.es/bitstreams/e86a8511-6a8c-4366-8dbf-8b7d85a4b764/download
File
MD5
4a2023e652ecbd1f8c896c6da4dbacf1
2308557
application/pdf
Delgado. 2017. Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide.pdf