2026-06-27T12:35:46Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/180482025-03-06T16:35:31Zcom_20.500.14352_14col_20.500.14352_15

Khodarahmi, Reza Torrente Rodríguez, Rebeca Magnolia Ruiz-Valdepeñas Montiel, Víctor Campuzano Ruiz, Susana Pedrero Muñoz, María Farchado, Meryem Vargas, Eva Manuel De Villena Rueda, Francisco Javier Garranzo Asensio, María Barderas Manchado, Rodrigo Pingarrón Carrazón, José Manuel 2023-06-17T22:05:33Z 2023-06-17T22:05:33Z 2017 1932-6203 10.1371/journal.pone.0175056 https://hdl.handle.net/20.500.14352/18048 https://doi.org/10.1371/journal.pone.0175056 The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates. eng https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates journal article