2026-06-29T08:13:50Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/189702025-07-15T12:34:55Zcom_20.500.14352_14col_20.500.14352_15
Characterization of a new toxin from the entomopathogenic
fungus Metarhizium anisopliae: the ribotoxin anisoplin
Olombrada, Miriam
Medina, Pilar
Budia, Flor
Gavilanes, José G.
Martínez Del Pozo, Álvaro
García Ortega, Lucía
577.1
hirsutellin
insecticide
ribonuclease
ribotoxin
sarcin.
Bioquímica (Química)
Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against target ribosomes and suggested as potential insecticides. Here, we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are on perspective.
Ministerio de Economía y Competitividad (MINECO)
Universidad Complutense de Madrid
Sección Deptal. de Bioquímica y Biología Molecular (Biológicas)
Depto. de Bioquímica y Biología Molecular
Fac. de Ciencias Biológicas
Fac. de Ciencias Químicas
FALSE
submitted
2023-06-17T23:53:16Z
2023-06-17T23:53:16Z
2016-09
journal article
https://hdl.handle.net/20.500.14352/18970
1437-4315
10.1515/hsz-2016-0119
eng
BFU2012-32404 and AGL2010-22196-C02-02
ESFUNPROT-UCM
restricted access
application/pdf
De Gruyter