2026-06-28T10:36:49Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/191762025-03-06T16:36:50Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Pedrero Muñoz, María author Manuel De Villena Rueda, Francisco Javier author Muñoz San Martín, Cristina author Campuzano Ruiz, Susana author Garranzo Asensio, María author Barderas Manchado, Rodrigo author Pingarrón Carrazón, José Manuel author 2016-11-21 An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation. 2079-6374 10.3390/bios6040056 https://hdl.handle.net/20.500.14352/19176 https://doi.org/10.3390/bios6040056 https://www.mdpi.com/2079-6374/6/4/56 Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers