2026-06-28T10:08:49Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/194562024-11-07T16:46:22Zcom_20.500.14352_14col_20.500.14352_21

00925njm 22002777a 4500 dc Gil Serna, Jessica author Patiño Álvarez, Aurora Belén author Jurado, Miguel author Mirete Castañeda, Salvador author Vázquez Estévez, María Covadonga Inmaculada author González Jaén, María Teresa author 2017 Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions. 978-1-4939-6705-6 (Print) 978-1-4939-6707-0 (Online) 10.1007/978-1-4939-6707-0_8 https://hdl.handle.net/20.500.14352/19456 https://link.springer.com/protocol/10.1007%2F978-1-4939-6707-0_8 Targeting conserved genes in fusarium species