2026-06-27T22:25:52Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/232532023-08-26T08:38:46Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Sánchez Elordi, Elena author Baluska, Frantisek author Echevarría, Clara author Vicente Córdoba, Carlos author Legaz González, María Estrella 2023-06-18T05:45:21Z 2023-06-18T05:45:21Z 2016 0176-1617 10.1016/j.jplph.2016.05.022 https://hdl.handle.net/20.500.14352/23253 https://www.journals.elsevier.com/journal-of-plant-physiology/ Microtubules (MTs) are involved in the germination of Sporisorium scitamineum teliospores. Resistant varieties of sugar cane plants produce defence glycoproteins that prevent the infection of the plants by the filamentous fungi Sporisorium scitamineum. Here, we show that a fraction of these glycoproteins pre-vents the correct arrangement of MTs and causes nuclear fragmentation defects. As a result, nuclei cannotcorrectly migrate through the growing hyphae, causing germinative failure. Arginase activity contained in defence glycoproteins is already described for preventing fungal germination. Now, its enzymatically active form is presented as a link between the defensive capacity of glycoproteins and the MT disor-ganization in fungal cells. Active arginase is produced in healthy and resistant plants; conversely, it is not detected in the juice from susceptible varieties, which explains why MT depolarization, nuclear dis-organization as well as germination of teliospores are not significantly affected by glycoproteins from non-resistant plants. Our results also suggest that susceptible plants try to increase their levels of arginase after detecting the presence of the pathogen. However, this signal comes “too late” and such defensive mechanism fails. eng Defence sugarcane glycoproteins disorganize microtubules andprevent nuclear polarization and germination of Sporisoriumscitamineum teliospores journal article URL https://docta.ucm.es/bitstreams/60acb206-18b9-43fb-83ea-bbfbd1463ec9/download File MD5 1dd9c60e46806b6f7202f069412a4258 13684619 application/pdf Vicente. 2016 Defence sugarcane glycoproteins disorganize microtubules and prevent nuclear polarization and germination of Sporisorium scitamineum teliospores.pdf