2026-06-27T16:04:19Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/234672025-05-06T10:52:49Zcom_20.500.14352_14col_20.500.14352_15

Maddock, Simon T. Briscoe, Andrew G. Wilkinson, Mark Waeschenbach, Andrea San Mauro Martín, Diego Day, Julia J. Littlewood, D. Tim J. Foster, Peter G. Nussbaum, Ronald A. Gower, David J. 2023-06-18T05:50:54Z 2023-06-18T05:50:54Z 2016-06 Maddock ST, Briscoe AG, Wilkinson M, Waeschenbach A, San Mauro D, Day JJ, et al. (2016) Next-Generation Mitogenomics: A Comparison of Approaches Applied to Caecilian Amphibian Phylogeny. PLoS ONE 11(6): e0156757. doi:10.1371/ journal.pone.0156757 1932-6203 10.1371/journal.pone.0156757 https://hdl.handle.net/20.500.14352/23467 https://doi.org/10.1371/journal.pone.0156757 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0156757 Mitochondrial genome (mitogenome) sequences are being generated with increasing speed due to the advances of next-generation sequencing (NGS) technology and associated analytical tools. However, detailed comparisons to explore the utility of alternative NGS approaches applied to the same taxa have not been undertaken. We compared a ‘traditional’ Sanger sequencing method with two NGS approaches (shotgun sequencing and non-indexed, multiplex amplicon sequencing) on four different sequencing platforms (Illumina’s HiSeq and MiSeq, Roche’s 454 GS FLX, and Life Technologies’ Ion Torrent) to produce seven (near-) complete mitogenomes from six species that form a small radiation of caecilian amphibians from the Seychelles. The fastest, most accurate method of obtaining mitogenome sequences that we tested was direct sequencing of genomic DNA (shotgun sequencing) using the MiSeq platform. Bayesian inference and maximum likelihood analyses using seven different partitioning strategies were unable to resolve compellingly all phylogenetic relationships among the Seychelles caecilian species, indicating the need for additional data in this case. eng https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Next-Generation Mitogenomics:A Comparison of Approaches Applied to Caecilian Amphibian Phylogeny journal article