2026-06-29T02:08:05Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/239292023-08-26T10:43:58Zcom_20.500.14352_14col_20.500.14352_15
Anti-Human Endoglin (hCD105) Immunotoxin—Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1
Barriuso, Begoña
Antolín, Pilar
Arias, F. Javier
Girotti, Alessandra
Jimenez López, Pilar
Córdoba Díaz, Manuel
Córdoba Díaz, Damián
Girbés Juan, Tomás
615.4
Recombinant musarmin
Endoglin
CD105
44G4
Anti-endoglin monoclonal antibody
Anti-tumor therapy
Cancer therapy
Tecnología farmaceútica
Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)— containing recombinant musarmin 1 (single chain ribosome- inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 ˆ 10´10 to 10´9 M.
Junta de Castilla y León
Depto. de Farmacia Galénica y Tecnología Alimentaria
Fac. de Farmacia
TRUE
pub
2023-06-18T06:08:03Z
2023-06-18T06:08:03Z
2016-06-10
journal article
https://hdl.handle.net/20.500.14352/23929
2072-6651
10.3390/toxins8060184
eng
CICYT BIO98-0727
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
open access
application/pdf