2026-08-20T06:02:09Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/246772025-07-16T17:50:49Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Moleres, Javier author Santos López, Alfonso author Lázaro, Isidro author Labairu, Javier author Prat, Cristina author Ardanuy, Carmen author González Zorn, Bruno author Aragón, Virginia author Garmendia, Junkal 2023-06-18T06:57:43Z 2023-06-18T06:57:43Z 2015 1098-5336 10.1128 /AEM.03865-14 https://hdl.handle.net/20.500.14352/24677 http://dx.doi.org/10.1128 /AEM.03865-14 Haemophilus parasuis, the causative agent of Glässer's disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer's disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria. eng Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs journal article URL https://docta.ucm.es/bitstreams/11ab099d-351a-409d-ade3-2a8855a25be1/download File MD5 2833c3ec5c3c06348c8f0a6db07621bc 308742 application/pdf Appl. Environ. Microbiol.-2015-Moleres-3255-67.pdf