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oai:docta.ucm.es:20.500.14352/340012025-03-26T00:50:03Zcom_20.500.14352_14col_20.500.14352_15

Wojtusik, Mateusz Rodríguez Martín, Alberto Ripoll Morales, Vanessa Santos Mazorra, Victoria Eugenia García, Jose L. García-Ochoa Soria, Félix 2023-06-19T13:33:13Z 2023-06-19T13:33:13Z 2015 8756-7938 (Print) 1520-6033 (Online) 10.1016/j.btre.2014.12.010 https://hdl.handle.net/20.500.14352/34001 http://www.sciencedirect.com/science/article/pii/S2215017X14000666 Production of 1,3-propanediol from glycerol using Klebsiella oxytoca NRRL-B199 has been studied. Medium composition has been optimized by means of a statistical design based on the Taguchi method. Strong influences of glycerol and phosphate concentrations have been detected on biomass and product yields. Other factors, such as magnesium concentration and K:Na ratio, have shown a small influence on both responses, biomass and product concentrations. An optimized medium composition has been proposed, leading to a final 1,3-propanediol concentration of 12.4 g/L with a selectivity of 72% with respect to glycerol consumed at shaken bottle-scale. Once the medium composition had been optimized, the scale-up from shaken bottles to STBR was conducted. Several experiments in a 2 L STBR have been conducted in order to determine the best operating conditions concerning temperature and agitation. Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained. eng https://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access Atribución-NoComercial-SinDerivadas 3.0 España 1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions journal article