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1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions Wojtusik, Mateusz Rodríguez Martín, Alberto Ripoll Morales, Vanessa Santos Mazorra, Victoria Eugenia García, Jose L. García-Ochoa Soria, Félix 66.0 3-Propanediol production Klebsiella oxytoca Medium composition Operating conditions Scale-up Ingeniería química 3303 Ingeniería y Tecnología Químicas Production of 1,3-propanediol from glycerol using Klebsiella oxytoca NRRL-B199 has been studied. Medium composition has been optimized by means of a statistical design based on the Taguchi method. Strong influences of glycerol and phosphate concentrations have been detected on biomass and product yields. Other factors, such as magnesium concentration and K:Na ratio, have shown a small influence on both responses, biomass and product concentrations. An optimized medium composition has been proposed, leading to a final 1,3-propanediol concentration of 12.4 g/L with a selectivity of 72% with respect to glycerol consumed at shaken bottle-scale. Once the medium composition had been optimized, the scale-up from shaken bottles to STBR was conducted. Several experiments in a 2 L STBR have been conducted in order to determine the best operating conditions concerning temperature and agitation. Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained. Ministerio de Ciencia, Innovación y Universidades (España) Universidad Complutense de Madrid Depto. de Ingeniería Química y de Materiales Fac. de Ciencias Químicas TRUE pub 2023-06-19T13:33:13Z 2023-06-19T13:33:13Z 2015 journal article https://hdl.handle.net/20.500.14352/34001 8756-7938 (Print) 1520-6033 (Online) 10.1016/j.btre.2014.12.010 eng CTQ2010-11765-E CTQ2010-15460 CTQ2011- 12725-E GR35/10-A-910134 Atribución-NoComercial-SinDerivadas 3.0 España https://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access application/pdf Elsevier