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00925njm 22002777a 4500 dc Porrero, M Concepción author Mentaberre, Gregorio author Sánchez, Sergio author Fernández Llario, Pedro author Casas Díaz, Encarna author Mateos García, Ana Isabel author Vidal, Dolors author Lavín, Santiago author Domínguez Rodríguez, Lucas José author Fernández-Garayzábal Fernández, José Francisco author 2014-08 The presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P = 0.057). A higher proportion of isolates (P = 0.000) were recovered from nasal samples (78.51%) than skin samples (21.49%), but the 9.26% of red deer and 18.25% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59%) in Iberian ibex, t548 and t11212 (15.79% and 14.04%) in red deer, and t3750 (36.11%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72%), ST425 for red deer (29.82%), and ST2328 for wild boar (42.36%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P = 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species. 1098-5336 10.1128/AEM.00647-14 https://hdl.handle.net/20.500.14352/35276 http://aem.asm.org/content/early/2014/06/03/AEM.00647-14 Carriage of Staphylococcus aureus by free-living wild animals in Spain