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oai:docta.ucm.es:20.500.14352/355252024-09-03T14:09:03Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Ojeda Fernández, Irene author Moreno Guzmán, María author González Cortés, Araceli author Yáñez-Sedeño Orive, Paloma author Pingarrón Carrazón, José Manuel 2023-06-19T15:11:50Z 2023-06-19T15:11:50Z 2014 Ojeda Fernández, I., Moreno Guzmán, M., González Cortés, A. et al. «Electrochemical Magnetoimmunosensor for the Ultrasensitive Determination of Interleukin-6 in Saliva and Urine Using Poly-HRP Streptavidin Conjugates as Labels for Signal Amplification». Analytical and Bioanalytical Chemistry, vol. 406, n.o 25, octubre de 2014, pp. 6363-71. DOI.org (Crossref), https://doi.org/10.1007/s00216-014-8055-6. 1618-2642 10.1007/s00216-014-8055-6 https://hdl.handle.net/20.500.14352/35525 1618-2650 https//doi.org/10.1007/s00216-014-8055-6 https://link.springer.com/article/10.1007/s00216-014-8055-6 A novel magnetoimmunosensor design for interleukin-6 (IL-6) which involved the covalent immobilization of anti-IL-6 antibodies onto carboxyl-functionalized magnetic microparticles and a sandwich-type immunoassay with signal amplification using poly-HRP-streptavidin conjugates is reported. All the variables concerning the preparation and the electroanalytical performance of the immunosensor were optimized. The use of poly-HRP-strept conjugates as enzymatic labels instead of conventional HRP-strept allowed enhanced signal-to-blank current ratios to be obtained. A linear calibration plot between the measured steady-state current and the log of IL-6 concentration was achieved in the 1.75 to 500 pg/mL range, which was not feasible when using HRP-strep as label. A limit of detection of 0.39 pg/mL IL-6 was obtained. The antiIL-6-MB conjugates exhibited an excellent storage stability providing amperometric responses with no significant loss during at least 36 days. The magnetoimmunosensor showed also an excellent selectivity against potentially interfering substances. The immunosensor was used to determine IL-6 in urine samples spiked at three different concentration levels with clinical relevance. Moreover, IL-6 was measured in three different saliva samples corresponding to a periodontitis patient, a smoker volunteer, and a non-smoker volunteer. The obtained results were statistically in agreement with those provided by a commercial ELISA kit. eng Electrochemical magnetoimmunosensor for the ultrasensitive determination of interleukin-6 in saliva and urine using poly-HRP streptavidin conjugates as labels for signal amplification journal article URL https://docta.ucm.es/bitstreams/cc6da5a2-3e1c-4e2f-a398-6dd7be4960d6/download File MD5 404babd5125435658546d1ccc7229a0e 1990291 application/pdf 2014 ABC IL6 proof print (1).pdf URL https://docta.ucm.es/bitstreams/29cf150d-ee9e-4040-8e0c-bd1d9275e340/download File MD5 ce6511ea0bbb9ba4f1b0534d43c07fb1 37998 text/plain 2014 ABC IL6 proof print (1).pdf.txt