2026-06-28T04:47:53Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/419642024-09-23T17:16:22Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Alvarez García, Elisa
author
Diago Navarro, Elizabeth
author
Herrero Galán, Elías
author
García Ortega, Lucía
author
López Villarejo, Juan
author
Olmo López, Nieves
author
Díaz Orejas, Ramón
author
Gavilanes, José G.
author
Martínez Del Pozo, Álvaro
2023-06-20T00:06:05Z
2023-06-20T00:06:05Z
2011
1570-9639
10.1016/j.bbapap.2011.06.017
https://hdl.handle.net/20.500.14352/41964
http://www.elsevier.com/wps/find/journaldescription.cws_home/635707/description#description
Fungal ribotoxins are toxic secreted ribonucleases that cleave a conserved single phosphodiester bond located at the sarcin/ricin loop of the larger rRNA. This cleavage inactivates ribosomes leading to protein biosynthesis inhibition and cell death. It has been proposed that interactions other than those found at the active site of ribotoxins are needed to explain their exquisite specific activity. The study presented shows the ability of a catalytically inactive α-sarcin mutant (H137Q) to bind eukaryotic ribosomes and interfere with in vitro protein biosynthesis. The results obtained are compatible with previous observations that α-sarcin can promote cell death by a mechanism that is independent of rRNA cleavage, expanding the potential set of activities performed by this family of toxins.
spa
The ribonucleolytic activity of the ribotoxin α-sarcin is not essential for in vitro protein biosynthesis inhibition
journal article
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BBAPRO-11-147R1_RevisadoEnviado_eprintsComplutense.pdf
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85bd520cbe2b4c9ba32eb5ea47317572
39092
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BBAPRO-11-147R1_RevisadoEnviado_eprintsComplutense.pdf.txt