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oai:docta.ucm.es:20.500.14352/438722024-04-16T14:08:14Zcom_20.500.14352_14col_20.500.14352_15

Sanz Santamaría, Ana Belén García Sánchez, Raúl Rodríguez Peña, José Manuel Díez Muñiz, Sonia Nombela Cano, César Peterson, Craig L. Arroyo Nombela, Francisco Javier 2023-06-20T03:33:36Z 2023-06-20T03:33:36Z 2012-05-23 1939-4586 10.1091/mbc.E12-04-0278 https://hdl.handle.net/20.500.14352/43872 http://www.molbiolcell.org/content/23/14/2805 In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress. eng https://creativecommons.org/licenses/by-nc-sa/4.0/ open access Attribution-NonCommercial-ShareAlike 4.0 International Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway. journal article