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00925njm 22002777a 4500 dc Álvarez, Granada author Visitación Bartolomé, M. author Miana, María author Jurado López, Raquel author Martín, Rubén author Zuluaga Arias, María del Pilar author Martínez Martínez, Ernesto author Nieto, María Luisa author Álvarez Sala, Luis A. author Millán, Jesús author Lahera Julia, Vicente author Cachofeiro Ramos, María Victoria author 2012 The effect of adiponectin and leptin on the proliferation of the human microvascular endothelial cell line (HMEC-1) was studied in the absence or presence of fetal bovine serum (FBS). The participation of extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt (PI-3K/Akt) pathways in this effect were evaluated. We studied the effect of both adipokines on the motility, mitosis, proliferation and cell death processes of HMEC-1 cells using live-cell imaging techniques. Adiponectin but not leptin further increased the proliferative effect induced by FBS on HMEC-1. This effect seems to be the consequence of an increase in the mitotic index in adiponectin-treated cells when compared to untreated ones. The presence of either the mitogen-activated protein kinase (MAPK) inhibitor (PD98059), or PI-3K inhibitor (LY294002), reduced the effect of adiponectin in a dose-dependent manner. Neither adipokine was able to affect HMEC-1 proliferation in FBS-free conditions. Duration of mitosis, cell motility and the cell death process were similar in all conditions. These data suggest that adiponectin and leptin exert different effects on endothelial cell function. Adiponectin was able to potentiate proliferation of HMEC-1. This effect involves the activation of both PI3-K/Akt and ERK/MAPK pathways. However, it seems to exert minimal effects on HMEC-1 function in the case of leptin Álvarez, G., Visitarión Bartolomé, M., Miana, M. et al. «The Effects of Adiponectin and Leptin on Human Endothelial Cell Proliferation: A Live-Cell Study». Journal of Vascular Research, vol. 49, n.o 2, 2012, pp. 111-22. DOI.org (Crossref), https://doi.org/10.1159/000332332. 1018-1172 10.1159/000332332 https://hdl.handle.net/20.500.14352/44609 https//doi.org/10.1159/000332332 http://www.karger.com/Article/FullText/332332 The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study