2026-06-08T03:46:31Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/446092024-09-23T13:26:43Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Álvarez, Granada
author
Visitación Bartolomé, M.
author
Miana, María
author
Jurado López, Raquel
author
Martín, Rubén
author
Zuluaga Arias, María del Pilar
author
Martínez Martínez, Ernesto
author
Nieto, María Luisa
author
Álvarez Sala, Luis A.
author
Millán, Jesús
author
Lahera Julia, Vicente
author
Cachofeiro Ramos, María Victoria
2023-06-20T03:52:55Z
2023-06-20T03:52:55Z
2012
Álvarez, G., Visitarión Bartolomé, M., Miana, M. et al. «The Effects of Adiponectin and Leptin on Human Endothelial Cell Proliferation: A Live-Cell Study». Journal of Vascular Research, vol. 49, n.o 2, 2012, pp. 111-22. DOI.org (Crossref), https://doi.org/10.1159/000332332.
1018-1172
10.1159/000332332
https://hdl.handle.net/20.500.14352/44609
https//doi.org/10.1159/000332332
http://www.karger.com/Article/FullText/332332
The effect of adiponectin and leptin on the proliferation of the human microvascular endothelial cell line (HMEC-1) was studied in the absence or presence of fetal bovine serum (FBS). The participation of extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase/Akt (PI-3K/Akt) pathways in this effect were evaluated. We studied the effect of both adipokines on the motility, mitosis, proliferation and cell death processes of HMEC-1 cells using live-cell imaging techniques. Adiponectin but not leptin further increased the proliferative effect induced by FBS on HMEC-1. This effect seems to be the consequence of an increase in the mitotic index in adiponectin-treated cells when compared to untreated ones. The presence of either the mitogen-activated protein kinase (MAPK) inhibitor (PD98059), or PI-3K inhibitor (LY294002), reduced the effect of adiponectin in a dose-dependent manner. Neither adipokine was able to affect HMEC-1 proliferation in FBS-free conditions. Duration of mitosis, cell motility and the cell death process were similar in all conditions. These data suggest that adiponectin and leptin exert different effects on endothelial cell function. Adiponectin was able to potentiate proliferation of HMEC-1. This effect involves the activation of both PI3-K/Akt and ERK/MAPK pathways. However, it seems to exert minimal effects on HMEC-1 function in the case of leptin
eng
The effects of adiponectin and leptin on human endothelial cell proliferation: a live-cell study
journal article
URL
https://docta.ucm.es/bitstreams/dab4163d-2c2e-4041-bea6-88761a91bc2d/download
File
MD5
8c934ce2a316f1ea40b9d195404c1dad
822551
application/pdf
Zuluaga06oficial.pdf