2026-06-27T12:31:56Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/44842024-04-16T15:17:23Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author García Fontecha, Ana author González Ortiz, Blanca author Harvey, Katherine author Izquierdo Barba, Isabel author Vallet Regí, María Dulce Nombre 2023-06-16T14:16:36Z 2023-06-16T14:16:36Z 2021-10-07 1387-1811 10.1016/j.micromeso.2021.111489 https://hdl.handle.net/20.500.14352/4484 https://doi.org/10.1016/j.micromeso.2021.111489 https://www.ucm.es/valletregigroup Bacterial biofilms can initiate chronic infections that become difficult to eradicate. There is an unmet need for effective therapeutic strategies that control and inhibit the growth of these biofilms. Herein, light sensitive mesoporous silica nanoparticles (MSNs) with photothermal (PTT) and antimicrobial combined capabilities have been developed. These nanosystems have high therapeutic potential to affect the bacterial biofilm architecture and subsequently inhibit its growth. Nucleation of gold nanorods followed by the growth of a silica shell leads to a core@shell design (AuNR@MSN) with PTT properties. Incorporation of nitrosothiol groups (-SNO) with a heat liable linker, enables an enhanced nitric oxide release upon photothermal stimulation with near infrared radiation. Further loading of an antimicrobial molecule such as the levofloxacin (LEVO) antibiotic creates a unique nanoassembly with potential therapeutic efficacy against Staphylococcus aureus bacterial biofilms. A dispersion rate of the bacterial biofilm was evident when light stimuli is applied because impregnation of the nitrosothiol functionalized nanosystem with the antibiotic LEVO led to ca. 30% reduction but its illumination with near infrared (NIR) irradiation showed a biofilm reduction of ca. 90%, indicating that localized antimicrobial exposure and PTT improves the therapeutic efficacy. These findings envision the conception of near-infrared-activated nanoparticle carriers capable of combined therapy upon NIR irradiation, which enables photothermal therapy, together with the release of levofloxacin and nitric oxide to disrupt the integrity of bacterial biofilms and achieve a potent antimicrobial therapy. eng Atribución-NoComercial-SinDerivadas 3.0 España Effective reduction of biofilm through photothermal therapy by gold core@shell based mesoporous silica nanoparticles. journal article URL https://docta.ucm.es/bitstreams/116dbe4d-1fcc-4bc5-a9de-604a0b9f78c8/download File MD5 69e4bb04f1de28bfdc36be221dc86665 1602704 application/pdf pagination_MICMAT_111489.pdf URL https://docta.ucm.es/bitstreams/d5b3ebef-eb12-401c-b846-a7f155cf5fef/download File MD5 7b95a774e3d11c5a80aa854fedd38f54 58816 text/plain pagination_MICMAT_111489.pdf.txt