2026-06-27T10:23:11Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/451492024-07-04T16:14:08Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Andrés Guerrero, Vanesa author Molina Martínez, Irene Teresa author Peral Cerda, María Asunción author Heras Polo, Beatriz De Las author Pintor Just, Jesús Jerónimo author Herrero Vanrell, María Del Rocío 2023-06-20T04:18:21Z 2023-06-20T04:18:21Z 2011-03 1552-5783 10.1167/iovs.10-6099 https://hdl.handle.net/20.500.14352/45149 http://iovs.arvojournals.org/article.aspx?articleid=2126793&resultClick=1 Purpose.: 5-Methoxy-carbonylamino-N-acetyltryptamine (5-MCA-NAT, a melatonin receptor agonist) produces a clear intraocular pressure (IOP) reduction in New Zealand White rabbits and glaucomatous monkeys. The goal of this study was to evaluate whether the hypotensive effect of 5-MCA-NAT was enhanced by the presence of cellulose derivatives, some of them with bioadhesive properties, as well as to determine whether these formulations were well tolerated by the ocular surface. Methods.: Formulations were prepared with propylene glycol (0.275%), carboxymethyl cellulose (CMC, 0.5% and 1.0%) of low and medium viscosity and hydroxypropylmethyl cellulose (0.3%). Quantification of 5-MCA-NAT (100 μM) was assessed by HPLC. In vitro tolerance was evaluated by the MTT method in human corneal-limbal epithelial cells and normal human conjunctival cells. In vivo tolerance was analyzed by biomicroscopy and specular microscopy in rabbit eyes. The ocular hypotensive effect was evaluated measuring IOP for 8 hours in rabbit eyes. Results.: All the formulations demonstrated good in vitro and in vivo tolerance. 5-MCA-NAT in CMC medium viscosity 0.5% was the most effective at reducing IOP (maximum IOP reduction, 30.27%), and its effect lasted approximately 7 hours. Conclusions.: The hypotensive effect of 5-MCA-NAT was increased by using bioadhesive polymers in formulations that are suitable for the ocular surface and also protective of the eye in long-term therapies. The use of 5-MCA-NAT combined with bioadhesive polymers is a good strategy in the treatment of ocular hypertension and glaucoma. eng Atribución-NoComercial-SinDerivadas 3.0 España The Use of Mucoadhesive Polymers to Enhance the Hypotensive Effect of a Melatonin Analogue, 5-MCA-NAT, in Rabbit Eyes journal article URL https://docta.ucm.es/bitstreams/b3b44b8a-3575-443d-a7d9-62800ec5b823/download File MD5 b7d8de9fe2ce77b8bcaf7a052991c5bf 480218 application/pdf use od mucoadhesive_iovs-2011.pdf