2026-07-23T04:54:31Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/451832024-06-12T13:20:54Zcom_20.500.14352_14col_20.500.14352_15

Utilization of lactose and presence of the phospho-β-galactosidase (lacG) gene in Lactococcus garvieae isolates from different sources Aguado Urda, Mónica Blanco Gutiérrez, María Del Mar Cutuli Simón, María Teresa Aspiroz, Carmen Tejedor, José L Gibello Prieto, Alicia Fernández-Garayzábal Fernández, José Francisco Lactococcus garvieae lactose metabolism lacG gen Veterinaria 3109 Ciencias Veterinarias This study evaluates the utilization of lactose (Lac) and the presence of the phospho-β-galactosidase (lacG) gene as markers for distinguishing between fish (Lac-/lacG-) and dairy isolates (Lac+/lacG+) of Lactococcus garvieae, using a panel of L. garvieae isolates from different sources. None of the fish isolates produced acid from lactose (Lac-), however Lac-/lacG- isolates were observed in pigs, cows, birds and humans. Most of the dairy isolates (77.8%) were Lac+/lacG+, but some dairy isolates did not produce acid from this sugar. Data in the present study show that the ability to metabolize lactose and the presence of the lacG gene are heterogeneously scattered among L. garvieae isolates of different sources. Therefore, the use of these criteria as markers to differentiate between L. garvieae isolates of dairy and fish origin should be considered with caution. Ministerio de Ciencia e Innovación (MICINN) Centro de Vigilancia Sanitaria Veterinaria (VISAVET) TRUE pub 2023-06-20T04:20:20Z 2023-06-20T04:20:20Z 2010-12 journal article https://hdl.handle.net/20.500.14352/45183 1618-1905 10.2436/20.1501.01.125 eng AGL2009- 12447 Atribución-NoComercial-CompartirIgual 3.0 España https://creativecommons.org/licenses/by-nc-sa/3.0/es/ open access application/pdf Spanish Society for Microbiology