2026-08-20T06:58:04Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/451862023-12-04T16:34:55Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Palacios, Lucía author Vela Alonso, Ana Isabel author Molin, Kent author Fernández, Ana author Latre, Maria Victoria author Chacón, Gema author Falsen, Enevold author Fernández-Garayzábal Fernández, José Francisco author 2010-09 Eighteen Corynebacterium xerosis strains isolated from different animal clinical specimens were subjected to phenotypic and molecular genetic studies. On the basis of the results of the biochemical characterization, the strains were tentatively identified as C. xerosis. Phylogenetic analysis based on comparative analysis of the sequences of 16S rRNA and rpoB genes revealed that the 18 strains were highly related to C. xerosis, C. amycolatum, C. freneyi, and C. hansenii. There was a good concordance between 16S rRNA and partial rpoB gene sequencing results, although partial rpoB gene sequencing allowed better differentiation of C. xerosis. Alternatively, C. xerosis was also differentiated from C. freneyi and C. amycolatum by restriction fragment length polymorphism analysis of the 16S-23S rRNA gene intergenic spacer region. Phenotypic characterization indicated that besides acid production from D-turanose and 5-ketogluconate, 90% of the strains were able to reduce nitrate. The absence of the fatty acids C(14:0), C(15:0), C(16:1)omega 7c, and C(17:1)omega 8c can also facilitate the differentiation of C. xerosis from closely related species. The results of the present investigation demonstrated that for reliable identification of C. xerosis strains from clinical samples, a combination of phenotypic and molecular-biology-based identification techniques is necessary. 1098-660X 10.1128/JCM.02373-09 https://hdl.handle.net/20.500.14352/45186 http://jcm.asm.org/content/48/9/3138.full Characterization of some bacterial strains isolated from animal clinical materials and identified as Corynebacterium xerosis by molecular biological techniques