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oai:docta.ucm.es:20.500.14352/451902023-08-28T02:13:03Zcom_20.500.14352_14col_20.500.14352_15

Castellanos, Elena Aranaz Martín, Alicia De Buck, Jeroen 2023-06-20T04:20:43Z 2023-06-20T04:20:43Z 2010-04 1098-660X 10.1128/JCM.02484-09 https://hdl.handle.net/20.500.14352/45190 http://jcm.asm.org/content/48/4/1474.full High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family. eng restricted access Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus journal article