2026-06-28T09:50:14Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/451982024-04-10T14:17:02Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Pérez de Diego, Ana Cristina
author
Sánchez Cordón, Pedro José
author
Heras, Ana Isabel de las
author
Sánchez-Vizcaíno Rodríguez, José Manuel
2023-06-20T04:21:08Z
2023-06-20T04:21:08Z
2012
1537-744X
10.1100/2012/147158
https://hdl.handle.net/20.500.14352/45198
http://dx.doi.org/10.1100/2012/147158
The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes.
eng
Atribución 3.0 España
Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep
journal article
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https://docta.ucm.es/bitstreams/c70ff379-22a8-49fd-bc09-77b62726f47e/download
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501.pdf
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c7f9d7d714e6ebf1a68d384cd6dc7c97
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501.pdf.txt