2026-06-27T15:35:26Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/451982024-04-10T14:17:02Zcom_20.500.14352_14col_20.500.14352_15

Pérez de Diego, Ana Cristina Sánchez Cordón, Pedro José Heras, Ana Isabel de las Sánchez-Vizcaíno Rodríguez, José Manuel 2023-06-20T04:21:08Z 2023-06-20T04:21:08Z 2012 1537-744X 10.1100/2012/147158 https://hdl.handle.net/20.500.14352/45198 http://dx.doi.org/10.1100/2012/147158 The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes. eng https://creativecommons.org/licenses/by/3.0/es/ open access Atribución 3.0 España Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep journal article