2026-06-29T08:14:09Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/451982024-04-10T14:17:02Zcom_20.500.14352_14col_20.500.14352_15

Characterization of the immune response induced by a commercially available inactivated bluetongue virus serotype 1 vaccine in sheep Pérez de Diego, Ana Cristina Sánchez Cordón, Pedro José Heras, Ana Isabel de las Sánchez-Vizcaíno Rodríguez, José Manuel Veterinaria 3109 Ciencias Veterinarias The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4(+), CD8(+), and WC1(+)), CD25(+) regulatory cells, or CD14(+) monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14(+) monocytes, CD25(+) regulatory cells, and CD8(+) cytotoxic T lymphocytes. Ministerio de Ciencia e Innovación (MICINN) Unión Europea. FP6 Centro de Vigilancia Sanitaria Veterinaria (VISAVET) TRUE pub 2023-06-20T04:21:08Z 2023-06-20T04:21:08Z 2012 journal article https://hdl.handle.net/20.500.14352/45198 1537-744X 10.1100/2012/147158 eng AGL2009-13174-C02-01 BTVAC (44211) Atribución 3.0 España https://creativecommons.org/licenses/by/3.0/es/ open access application/pdf Hindawi Publishing Corporation