2026-06-27T11:22:24Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/519822024-09-12T15:20:53Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Ramírez Sebastián, Ana Isabel author Salazar Corral, Juan José author Hoz Montañana, María Rosa De author Rojas López, María Blanca author Ruiz Olivar, José Emilio author Tejerina Sánchez, María Teresa author Ramírez Sebastián, José Manuel author Triviño Casado, Alberto 2023-06-20T12:38:19Z 2023-06-20T12:38:19Z 2006-09-26 Ramírez Sebastián, A. I., Salazar Corral, J. J., Hoz Montañana, M. R. et al. «Macroglial and Retinal Changes in Hypercholesterolemic Rabbits after Normalization of Cholesterol Levels». Experimental Eye Research, vol. 83, n.o 6, diciembre de 2006, pp. 1423-38. DOI.org (Crossref), https://doi.org/10.1016/j.exer.2006.07.023. 0014-4835 10.1016/j.exer.2006.07.023 https://hdl.handle.net/20.500.14352/51982 http://dx.doi.org/10.1016/j.exer.2006.07.023 This study evaluates hypercholesterolemic rabbits, examining the retinal changes in Müller cells and astrocytes as well as their variations after a period of normal blood-cholesterol values induced by a standard diet. New Zealand rabbits were divided into three groups: G0, fed a standard diet; G1A, fed a 0.5% cholesterol-enriched diet for 8 months; and G1B, fed as G1A followed by standard diet for 6 months. Eyes were processed for transmission electron microscopy and immunohistochemistry (GFAP). While G1B resembled G0 more than did G1A, they shared alterations with G1A: a) as in G1A, Müller cells were GFAP+, filled spaces left by axonal degeneration, formed glial scars and their nuclei were displaced to the nerve-fibre layer. The area occupied by the astrocytes associated with the nerve-fibre bundles (AANFB) and by perivascular astrocytes (PVA) in G1A and G1B was significantly lower than in controls. However, no significant differences in PVA were found between G1A and G1B. In G1B, type I PVA was absent and replaced by hypertrophic type II cells; b) Bruch's membrane (BM) was thinner in G1B than in G1A; c) the retinal pigment epithelium (RPE) cytoplasm contained fewer lipids in G1B than in G1A; d) in G1A and G1B choriocapillaris and retinal vessel showed alterations with respect to G0; e) cell death and axonal degeneration in the retina were similar in G1A and G1B. The substitution of a hyperlipemic diet by a standard one normalizes blood-lipid levels. However, the persistence of damage at retinal vessels and BM-RPE could trigger chronic ischemia. eng Macroglial and retinal changes in hypercholesterolemic rabbits after normalization of cholesterol levels journal article URL https://docta.ucm.es/bitstreams/e9c0841f-0e5d-440a-bfd6-0cd8cf645a7b/download File MD5 a168c72af0f639521858db77e1acfc64 6359775 application/pdf macroglial and retinal-ExpmEyeResear-2006.pdf