2026-06-01T02:23:12Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/60202023-08-27T13:05:15Zcom_20.500.14352_14col_20.500.14352_15

Intensity-based axial localization at the quantum limit Řeháček, J. Stoklasa, Bohumil Koutný, Dominik Hradil, Zdenek Sánchez Soto, Luis Lorenzo 535 Fluorescence microscopy Óptica (Física) 2209.19 Óptica Física © 2019 American Physical Society. We thank Robert W. Boyd for helpful discussions. We acknowledge financial support from the Czech Science Foundation (Grant No. 18-04291S), the Technology Agency of the Czech Republic (Grant No. TE01020229), the Palacky University (Grant No. IGA_PrF_2019_007), and the Spanish MINECO (Grants No. FIS2015-67963-P and No. PGC2018-099183-B-I00). We derive fundamental precision bounds for single-point axial localization. For Gaussian beams, this ultimate limit can be achieved with a single intensity scan, provided the camera is placed at one of two optimal transverse detection planes. Hence, for axial localization there is no need of more complicated detection schemes. The theory is verified with an experimental demonstration of axial resolution 3 orders of magnitude below the classical depth of focus. Ministerio de Economía y Competitividad (MINECO) Czech Science Foundation Technology Agency of the Czech Republic Palacky University Depto. de Óptica Fac. de Ciencias Físicas TRUE pub 2023-06-16T15:15:43Z 2023-06-16T15:15:43Z 2019-11-07 journal article https://hdl.handle.net/20.500.14352/6020 0031-9007 10.1103/PhysRevLett.123.193601 eng (FIS2015-67963-P ; PGC2018-099183-B-I00) 18-04291S TE01020229 IGA_PrF_2019_007 open access application/pdf The American Physical Society