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Docta Complutense author Carpena Torres, Carlos author Pintor Just, Jesús Jerónimo author Pérez de Lara, María Jesús author Huete Toral, Fernando author Crooke Álvarez, Almudena author Pastrana Robles, Cristina author Carracedo Rodríguez, Juan Gonzalo 2023-06-16T15:18:43Z 2023-06-16T15:18:43Z 2020-05-30 Carpena-Torres, C., Pintor Just, J. J., Pérez de Lara, M. J. et al. «Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride». Journal of Ophthalmology, vol. 2020, mayo de 2020, pp. 1-10. DOI.org (Crossref), https://doi.org/10.1155/2020/7204951. 2090-004X 10.1155/2020/7204951 https://hdl.handle.net/20.500.14352/6300 https://doi.org/10.1155/2020/7204951 https://www.hindawi.com/journals/joph/2020/7204951/#copyright Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia (P < 0.001), corneal staining (P < 0.001), conjunctival hyperemia (P < 0.001), and levels of IL-6 mRNA (P = 0.005) compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia (P < 0.001), tear breakup time (P = 0.007), tear osmolarity (P < 0.001), density of goblet cells (P < 0.001), and height of mucin cloud (P < 0.001). Conclusions. )e topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days). eng Atribución 3.0 España Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride journal article URL https://docta.ucm.es/bitstreams/5382cdb1-4f42-4756-915d-2ba80c65f037/download File MD5 380a3c6087b3fab1f53112ad23dae6cb 2132182 application/pdf Croocke_2020_Hindawi_7204951.pdf