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oai:docta.ucm.es:20.500.14352/63622023-08-06T01:19:48Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Mota-Martorell, Natalia author Jové, Mariona author Pradas, Irene author Sánchez, Isabel author Gómez, José author Naudí, Alba author Barja de Quiroga, Gustavo author Pamplona, Reinald 2023-06-16T15:19:39Z 2023-06-16T15:19:39Z 2020-04-20 2213-2317, ESSN: 2213-2317 10.1016/j.redox.2020.101539 https://hdl.handle.net/20.500.14352/6362 https://www.sciencedirect.com/science/article/pii/S2213231720303281 Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species. eng Atribución-NoComercial-SinDerivadas 3.0 España Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity journal article URL https://docta.ucm.es/bitstreams/1b76170a-43ec-4bb3-8a1d-ae7b4aacd631/download File MD5 c202f0adef9beb171fa111cd2aed504c 1670584 application/pdf Mota-Martorell, Natalia et al. 2020. Low abundance of NDUFV2.....pdf URL https://docta.ucm.es/bitstreams/6638cf96-e038-4cd9-b09f-cd3f753d0962/download File MD5 2b3bea4735190b5963d65d469e2bd3d8 65140 text/plain Mota-Martorell, Natalia et al. 2020. Low abundance of NDUFV2.....pdf.txt