2026-06-29T02:49:03Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/63622023-08-06T01:19:48Zcom_20.500.14352_14col_20.500.14352_15
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity
Mota-Martorell, Natalia
Jové, Mariona
Pradas, Irene
Sánchez, Isabel
Gómez, José
Naudí, Alba
Barja de Quiroga, Gustavo
Pamplona, Reinald
591.1
577.112
599
Complex I
Droplet digital PCR
Longevity
Mammals
Mitochondria
NDUFV2 subunit
NDUFS4 subunit
VDAC
Western blot
Bioquímica (Biología)
Fisiología animal (Biología)
Mamíferos
2302 Bioquímica
2401.13 Fisiología Animal
2401.18 Mamíferos
Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
Ministerio de Economía y Competitividad (MINECO). Instituto de Salud Carlos III
Ministerio de Ciencia, Innovación y Universidades (MICIU)
Generalitat de Cataluña
Fondo Europeo de Desarrollo Regional(FEDER)
Depto. de Genética, Fisiología y Microbiología
Fac. de Ciencias Biológicas
TRUE
pub
2023-06-16T15:19:39Z
2023-06-16T15:19:39Z
2020-04-20
journal article
https://hdl.handle.net/20.500.14352/6362
2213-2317, ESSN: 2213-2317
10.1016/j.redox.2020.101539
eng
(PI14/00328)
(RTI2018-099200-B-I00)
(2017SGR696), SLT002/16/00250, PR [19] BIO MET 0155,AGAUR, ref 2018FI_B2_00104)
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
open access
application/pdf
Elsevier