2026-06-27T15:23:20Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/64452024-11-06T13:43:57Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Jiménez González, Sara author Marín Royo, Gema author Jurado López, Raquel author Bartolomé Pascual, María Visitación author Romero Miranda, Ana author Luaces Méndez, María author Islas, Fabián author Nieto, María Luisa author Martínez Martínez, Ernesto author Cachofeiro Ramos, María Victoria author 2020-02-17 The impact of the mitochondria-targeted antioxidant MitoQ was evaluated in the cardiac alterations associated with obesity. Male Wistar rats were fed either a high fat diet (HFD, 35% fat) or a standard diet (CT, 3.5% fat) for 7 weeks and treated with MitoQ (200 µM). The effect of MitoQ (5 nM) in rat cardiac myoblasts treated for 24 h with palmitic acid (PA, 200 µM) was evaluated. MitoQ reduced cardiac oxidative stress and prevented the development of cardiac fibrosis, hypertrophy, myocardial 18-FDG uptake reduction, and mitochondrial lipid remodeling in HFD rats. It also ameliorated cardiac mitochondrial protein level changes observed in HFD: reductions in fumarate hydratase, complex I and II, as well as increases in mitofusin 1 (MFN1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha, and cyclophilin F (cycloF). In vitro, MitoQ prevented oxidative stress and ameliorated alterations in mitochondrial proteins observed in palmitic acid (PA)-stimulated cardiac myoblasts: increases in carnitine palmitoyltransferase 1A, cycloF, and cytochrome C. PA induced phosphorylation of extracellular signal-regulated kinases and nuclear factor-κB p65. Therefore, the data show the beneficial effects of MitoQ in the cardiac damage induced by obesity and suggests a crosstalk between lipotoxicity and mitochondrial oxidative stress in this damage. 2073-4409 10.3390/cells9020451 https://hdl.handle.net/20.500.14352/6445 https://www.mdpi.com/2073-4409/9/2/451 The Crosstalk between Cardiac Lipotoxicity and Mitochondrial Oxidative Stress in the Cardiac Alterations in Diet-Induced Obesity in Rats