2026-06-07T23:45:09Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/64732024-07-05T16:27:29Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Martínez Águila, Alejandro author Fonseca Vázquez, Begoña author Pérez de Lara, María Jesús author Miras Portugal, María Teresa author Gómez Villafuertes, María Rosa author Carracedo Rodríguez, Juan Gonzalo author Pintor Just, Jesús Jerónimo 2023-06-16T15:21:34Z 2023-06-16T15:21:34Z 2020-07-29 1090-0535 https://hdl.handle.net/20.500.14352/6473 http://www.molvis.org/molvis/v26/530 Purpose: The objective of this study was to evaluate the changes in the melatoninergic receptors of DBA/2J and C57BL/6J mice with the development of glaucoma. DBA/2J mice are widely used to study the physiopathology of glaucoma due to the similarities of their eyes to human eyes and the resulting similarity in the development of their pathology. In addition, melatoninergic receptors are known for their control of intraocular pressure (IOP), reducing the production of aqueous humor; however, little is known about their relationship with the development of this pathology. Methods: mRNA expression of MT1, MT2, and GPR50 melatoninergic receptors was performed with quantitative real-time PCR. In addition, receptor expression was performed with immunohistochemical techniques on the ciliary processes. To further investigate the effect of melatonin and its analog 5-methoxycarbonylamino-N-acetyltryptamine (5-MCA-NAT) on IOP, animals were instilled with these compounds and the corresponding melatoninergic antagonists to assess their effect on IOP. Results: All melatoninergic receptor expression decayed with the development of the glaucomatous pathology in the DBA/2J mice, and was especially visible for the MT2 receptor. However, receptor expression was consistent in the C57BL/6J control mice across all ages investigated. Furthermore, IOP blockage was stronger with 4PPDOT (MT2 antagonist) only in the DBA/2J mice which suggests a correlation of this receptor with the development of the glaucomatous pathology in DBA/2J animals. Conclusions: Melatonin receptor expression decays with the development of the glaucomatous pathology. This implies that the physiologic hypotensive effect of endogenous melatonin reducing IOP is not possible. A solution for such changes in receptor expression is the exogenous application of melatonin or any of its analogs that permit the activation of the remaining melatonin receptors. eng Changes in melatonin receptor expression in a murine model of glaucoma journal article URL https://docta.ucm.es/bitstreams/cf90a6c8-01f0-4805-ae13-ef8947274f0d/download File MD5 09d659195fccf8a7bf156a7a5577057b 1510560 application/pdf Pintor_2020_mv-v26-530.pdf URL https://docta.ucm.es/bitstreams/e6cc8783-6390-4edc-835a-dc99411cadf0/download File MD5 51af87c658e15e2de4c99a48b52b07e3 42253 text/plain Pintor_2020_mv-v26-530.pdf.txt