2026-06-27T10:55:40Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/65442023-08-25T11:57:22Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Arroyo, Raquel author Khan, MerajAlam author Echaide Torreguitar, Mercedes author Pérez-Gil, Jesús author Palaniyar, Nades 2023-06-16T15:23:09Z 2023-06-16T15:23:09Z 2019-12-16 2399-3642 10.1038/s42003-019-0662-5 https://hdl.handle.net/20.500.14352/6544 https://www.nature.com/articles/s42003-019-0662-5 An exacerbated amount of neutrophil extracellular traps (NETs) can cause dysfunction of systems during inflammation. However, host proteins and factors that suppress NET formation (NETosis) are not clearly identified. Here we show that an innate immune collectin, pulmonary surfactant protein-D (SP-D), attenuates lipopolysaccharide (LPS)-mediated NETosis in human neutrophils by binding to LPS. SP-D deficiency in mice (Sftpd−/−) leads to excess NET formation in the lungs during LPS-mediated inflammation. In the absence of SP-D, NETs inhibit the surface-active properties of lung surfactant, essential to prevent the collapse of alveoli, the air breathing structures of the lungs. SP-D reverses NET-mediated inhibition of surfactant and restores the biophysical properties of surfactant. To the best of our knowledge, this study establishes for the first time that (i) SP-D suppresses LPS-mediated NETosis, (ii) NETs inhibit pulmonary surfactant function in the absence of SP-D, and (iii) SP-D can restore NET-mediated inhibition of the surfactant system. eng Atribución 3.0 España SP-D attenuates LPS-induced formation of human neutrophil extracellular traps (NETs), protecting pulmonary surfactant inactivation by NETs journal article URL https://docta.ucm.es/bitstreams/5e6041a6-cfe0-4efa-837e-78a8397e0696/download File MD5 91cc31f22522b1fdc01c5d095f3af7db 2475383 application/pdf Arroyo-DBBM-SP-D-attenuates.pdf URL https://docta.ucm.es/bitstreams/4df52621-b631-4803-9196-b27bc63c121a/download File MD5 4168fde5229c3e60a6842ebbbf4b1c5f 83521 text/plain Arroyo-DBBM-SP-D-attenuates.pdf.txt