2026-06-01T01:30:07Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/713282023-08-27T10:33:44Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Vargas Balbuena, Javier
author
Gómez Pedrero, José Antonio
author
Quiroga Mellado, Juan Antonio
author
Alonso Fernández, José
2023-06-22T10:40:44Z
2023-06-22T10:40:44Z
2022-01-31
1094-4087
10.1364/OE.444675
https://hdl.handle.net/20.500.14352/71328
http://dx.doi.org/10.1364/OE.444675
https://opg.optica.org
We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.
eng
Atribución 3.0 España
Enhancement of Cryo-EM maps by a multiscale tubular filter
journal article
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https://docta.ucm.es/bitstreams/400384af-bc7f-4f71-83e2-17e5d32335d4/download
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Vargas, Javier 06 libre+CC.pdf
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Vargas, Javier 06 libre+CC.pdf.txt