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Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples Luque Uria, Álvaro Peltomaa, Riikka Johanna Navarro Duro, Marina Fikacek, Sabrina Head, Trajen Deo, Sapna K. Daunert, Sylvia Benito Peña, María Elena Moreno Bondi, María Cruz 543 bioluminescence fumonisin B1 fusion proteins immunoassays luciferases Química analítica (Química) 2301 Química Analítica CRUE-CSIC (Acuerdos Transformativos 2022) The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method. Ministerio de Ciencia e Innovación (MICINN) Depto. de Química Analítica Fac. de Ciencias Químicas TRUE pub 2023-06-22T10:41:06Z 2023-06-22T10:41:06Z 2022-02-17 journal article https://hdl.handle.net/20.500.14352/71361 2629-2742 10.1002/anse.202100070 eng (RTI2018-096410-B-C21); (BES-2016-078137) Atribución-NoComercial-SinDerivadas 3.0 España https://creativecommons.org/licenses/by-nc-nd/3.0/es/ open access application/pdf Wiley-VCH