2026-06-01T01:26:35Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/713732024-10-03T13:42:29Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Valverde De La Fuente, Alejandro
author
Gordón Pidal, José María
author
Montero Calle, Ana
author
Arévalo Pérez, Beatriz
author
Serafín González-Carrato, Verónica
author
Calero, Miguel
author
Moreno Guzmán, María
author
López, Miguel Ángel
author
Escarpa, Alberto
author
Yáñez-Sedeño Orive, Paloma
author
Barderas Manchado, Rodrigo
author
Campuzano Ruiz, Susana
author
Pingarrón Carrazón, José Manuel
2023-06-22T10:41:15Z
2023-06-22T10:41:15Z
2022-02-01
2196-0216
10.1002/celc.202200055
https://hdl.handle.net/20.500.14352/71373
https://doi.org/10.1002/celc.202200055
Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values.
eng
Atribución-NoComercial 3.0 España
Paving the Way for Reliable Alzheimer’s Disease Blood
Diagnosis by Quadruple Electrochemical Immunosensing
journal article
URL
https://docta.ucm.es/bitstreams/5d079eca-d19a-4d53-ba6d-e729d1a07ba5/download
File
MD5
7a187fc4a054b746647bf274459c93da
1438489
application/pdf
ChemElectroChem - 2022 - Valverde - Paving the Way for Reliable Alzheimer s Disease Blood Diagnosis by Quadruple.pdf