2026-06-29T02:33:53Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/713732024-10-03T13:42:29Zcom_20.500.14352_14col_20.500.14352_15

Valverde De La Fuente, Alejandro Gordón Pidal, José María Montero Calle, Ana Arévalo Pérez, Beatriz Serafín González-Carrato, Verónica Calero, Miguel Moreno Guzmán, María López, Miguel Ángel Escarpa, Alberto Yáñez-Sedeño Orive, Paloma Barderas Manchado, Rodrigo Campuzano Ruiz, Susana Pingarrón Carrazón, José Manuel 2023-06-22T10:41:15Z 2023-06-22T10:41:15Z 2022-02-01 2196-0216 10.1002/celc.202200055 https://hdl.handle.net/20.500.14352/71373 https://doi.org/10.1002/celc.202200055 Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values. eng https://creativecommons.org/licenses/by-nc/3.0/es/ open access Atribución-NoComercial 3.0 España Paving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing journal article