2026-06-27T11:09:09Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/714332023-07-18T05:12:54Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Prat Vidal, Cristina author Crisóstomo, Verónica author Moscoso, Isabel author Báez Díaz, Claudia author Blanco Blázquez, Virginia author Gómez Mauricio, Guadalupe author Albericio, Guillermo author Aguilar, Susana author Fernández Santos, María Eugenia author Fernández Avilés, Francisco author Sánchez Margallo, Francisco M. author Bayes Genis, Antoni author Bernad, Antonio author 2021-12-28 Human cardiac progenitor cells (hCPC) are considered a good candidate in cell therapy for ischemic heart disease, demonstrating capacity to improve functional recovery after myocardial infarction (MI), both in small and large preclinical animal models. However, improvements are required in terms of cell engraftment and efficacy. Based on previously published reports, insulin-growth factor 1 (IGF-1) and hepatocyte growth factor (HGF) have demonstrated substantial cardioprotective, repair and regeneration activities, so they are good candidates to be evaluated in large animal model of MI. We have validated porcine cardiac progenitor cells (pCPC) and lentiviral vectors to overexpress IGF-1 (co-expressing eGFP) and HGF (co-expressing mCherry). pCPC were transduced and IGF1-eGFPpos and HGF-mCherrypos populations were purified by cell sorting and further expanded. Overexpression of IGF-1 has a limited impact on pCPC expression profile, whereas results indicated that pCPC-HGF-mCherry cultures could be counter selecting high expresser cells. In addition, pCPC-IGF1-eGFP showed a higher cardiogenic response, evaluated in co-cultures with decellularized extracellular matrix, compared with native pCPC or pCPC-HGF-mCherry. In vivo intracoronary co-administration of pCPC-IGF1-eGFP and pCPC-HFG-mCherry (1:1; 40 × 106/animal), one week after the induction of an MI model in swine, revealed no significant improvement in cardiac function. 2073-4409 10.3390/cells10102571 https://hdl.handle.net/20.500.14352/71433 https://doi.org/10.3390/cells10102571 https://www.mdpi.com/2073-4409/10/10/2571/htm Intracoronary Delivery of Porcine Cardiac Progenitor Cells Overexpressing IGF-1 and HGF in a Pig Model of Sub-Acute Myocardial Infarction